Regenerative Agricultural Development LLC
Texas
What lives beneath
contributes to
everything above.

— Barry Bonner

No single practice saves the soil. But living biology, combined with the right approach, might.

Ray, you have been
saying this for years.

You have stood in front of farmers across every state in this country and held up a jar of their own soil falling apart in water. You have shown them, as plainly as it can be shown, that the problem is not the weather, not the equipment, and not the seed. The problem is that we have taken the life out of the ground.

The microbial community that holds soil together, that moves water through it, that cycles nutrients and sequesters carbon, has been depleted across tens of millions of acres of American farmland. The farmer feels it as rising input costs and shrinking margins. The land shows it as compaction, runoff, and an inability to hold rainfall.

RAD Microbes is not here to tell you what you do not already know. We are here because of what you teach, and we are building the biological tools to deliver it. This document is the story of what we are doing, where we are in the process, and why we believe your life's work and ours are pointing at the same thing.

The mission of RAD Microbes is to stop the desertification of the Midwest by returning living microbial consortia to the soil at a scale and price point that makes them accessible to every farmer who needs them, in America and across the world.

Reduced fertilizer. Same corn.

A 2-acre trial in Prosper, Texas compared our Bacillus consortium applied with no additional NPK fertilizer against standard NPK-only control acres. The treated field carried residual fertility from the prior season. No fresh synthetic inputs were added. Soil was analysed via 16S rRNA sequencing by Environmental Genomics. Equivalent plant height and bushel yield. The control plot spent $460 per acre on fresh NPK fertilizer to get there. The microbial plots did not. We are not suggesting on the basis of these results that farmers should stop fertilizing. We are suggesting the biology changes what becomes possible over time.

786%
More nitrite oxidizers
Nitrite oxidizers, the bacteria that complete the nitrification cycle and lock nitrogen into plant-available form, were 786% higher in treated soil. The biology built its own fertility engine.
133%
More ammonia oxidizers
Ammonia oxidizers were 133% higher in microbial plots, driving nitrogen transformation efficiency that synthetic inputs can only substitute for, never replicate.
179%
More filamentous bacteria
Filamentous bacteria, responsible for soil aggregation, pore structure, and water retention, increased 179%. This is the biology that holds rain in the ground instead of letting it run off.
50%
Fewer opportunistic pathogens
Potential opportunistic pathogens were halved in treated soil. The fertilizer-only control showed enrichment of genera like Staphylococcus and Streptococcus, absent from the microbial plots.
5–15%
Yield improvement potential
Enrichment of nitrogen-fixing Microvirga and phosphate-solubilising Bacillus genera indicates 5-15% yield improvements through optimised nutrient management, on top of the input savings.
$460
Per acre: fertilizer cost, control
The conventionally fertilized control spent $460 per acre on synthetic NPK. The microbial plots achieved equivalent plant height and bushel yield on reduced inputs. That margin belongs to the farmer.
The Midwest is being
lost one harvest at a time.

Desertification is not a word most people associate with Iowa, Illinois, or Texas. It should be. Decades of synthetic fertilizer dependency, monoculture rotation, and tillage have stripped functional microbial life from vast areas of the most productive farmland on earth. What remains is increasingly degraded soil that cannot hold water, cannot cycle nutrients without chemical input, and cannot withstand the pressure that climate variability is placing on it.

Synthetic inputs treat the symptom. They do not restore the system. A field that sheds rainfall rather than drawing it down into the soil profile is a field moving in one direction. RAD Microbes is building the biological response to reverse that movement.

01
Water percolation
Restoring the soil's capacity to absorb and retain rainfall is a primary measurable outcome of our field programme. Runoff reduction is where degraded soil shows its recovery first.
02
Fertilizer independence
Returning functional microbial communities means farmers can begin reducing expensive synthetic inputs while maintaining yield. The biology does the work the chemistry has been substituting for.
03
Generational remediation
Where farmland has been worked hard across multiple generations, our microbial programme provides a structured remediation pathway, targeting measurable recovery within three to five years.
04
Accessible by design
Microbial tools have historically been priced beyond the reach of the farmers who need them most. RAD Microbes is building a model that works at the farm gate, including internationally.
The organisms we
are working with right now.

What follows is shared in confidence. RAD Microbes operates a five-organism liquid culture consortium delivered as a field inoculant. Each species was selected for a specific, documented role in the soil system. Together they cover nitrogen cycling, phosphate availability, soil structure, pathogen suppression, and chemical remediation. That is the full stack of what degraded agricultural land is missing.

This information is proprietary to Regenerative Agricultural Development LLC and is shared solely for the purpose of this conversation.

Active consortium: five organisms
Bacillus licheniformis Soil fertility and organic breakdown Produces exocellular enzymes that degrade complex organic compounds, unlocking nutrients that are present in the soil but unavailable to plants. This is the organism that begins restoring biological fertility in land that has been chemically managed for years. It reduces the need for applied nitrogen and phosphorus by making what is already there accessible.
Bacillus megaterium Phosphate solubilisation and root colonisation Solubilises phosphate and trace minerals locked in the soil matrix and produces extracellular polysaccharides that promote root colonisation and deeper nutrient uptake. Confirmed present and active at 2.6% relative abundance in our Prosper, Texas corn trial. Stronger root systems, better yield resilience, and lower dependency on applied phosphorus are the measurable outcomes.
Bacillus subtilis Pathogen suppression and water infiltration Secretes subtilisin enzymes and biosurfactants that suppress soil-borne pathogenic fungi and bacteria. It also degrades plant waxes and organic matter to improve soil percolation and water infiltration. This is the physical property Ray's jar-of-water test measures directly. This is the biology that determines whether rain soaks in or runs off.
Pseudomonas protegens, P. fluorescens, P. chlororaphis Chemical remediation and pesticide degradation Degrade recalcitrant pesticides and herbicides that persist in the soil from years of conventional management. This is the remediation layer: the organisms that address what decades of chemical application have left behind. In fields where herbicide carryover is suppressing microbial life, these species clear the way for the biological community to re-establish.
Trametes sp. White-rot fungi: persistent pollutant breakdown White-rot fungi with ligninolytic enzymes capable of degrading persistent pesticides and herbicides that bacterial species cannot reach. Where soils carry the most severe chemical burden, legacy herbicide use, industrial residues, heavy compaction history, Trametes provides the degradation capacity that bacteria alone cannot. It is the deep cleaning layer of the consortium.
Delivery and diagnostics

Applied as a liquid inoculant at planting. Verified by 16S rRNA sequencing.

The consortium is delivered as a liquid culture inoculant, applied at planting using standard agricultural protocols. Pre and post-treatment soil analysis via 16S rRNA sequencing by Environmental Genomics allows us to verify which organisms establish, at what abundance, and what functional shifts occur in the broader microbial community. No two sites receive an identical programme. The diagnostics determine the formulation.

Why we have access to
a multimillion dollar facility.

RAD Microbes does not operate in isolation. The organisation I am partnered with is an established leader in industrial bioremediation, specifically the response to petrochemical damage. When an oil company contaminates water, soil, or a waterway, this is the organisation that gets called in. Their formulations, their laboratory capability, and their field science have been deployed at some of the most demanding remediation environments on record, including events of national scale.

That infrastructure, those laboratories, that decades-deep formulation science, sits within our organisation. What they have not driven toward is agriculture. That is where RAD Microbes comes in. I lead the agricultural division. We are the ones taking that science into the field, into the farm, and into the hands of the farmer. The capability is theirs. The agricultural direction is ours entirely.

01
Industrial bioremediation at scale
Our parent organisation specialises in the biological response to petrochemical contamination. When oil and gas operations damage soil or water, they restore it. That is the scale of science and operational experience that sits behind our agricultural formulations.
02
Laboratory and formulation capability
Access to a multimillion dollar facility means our consortium development, testing, and quality assurance is not limited by resources. The biology is validated at a level most agricultural bioinoculant companies cannot access independently.
03
RAD Microbes drives the agriculture
Our partners have experience working with agricultural systems but have not pursued that market as a direction. That decision sits with us. We are the agricultural division. We determine the strategy, the trials, the farmer relationships, and the path to scale. The science is shared. The mission is ours.

Why this matters. Early-stage bioinoculant programmes typically face two constraints: the science is underdeveloped, or the resources to validate it are not there. RAD Microbes does not face either. The formulation science is deep and field-proven across extreme environments. The laboratory infrastructure is already in place. What we are building now is the agricultural application of that capability, and we are building it to last.

Fourteen documented
capabilities. One formulation.

Each of the following is attributable to one or more organisms within the consortium, supported by peer-reviewed science and our own field data. This is not a list of aspirational claims. It is a list of documented biological functions that occur when a healthy, diverse microbial community is present in the soil.

Atmospheric nitrogen fixation: Microvirga and Azospirillum genera fix atmospheric nitrogen directly, reducing dependency on applied nitrogen fertilizer.
Ammonia oxidation: Confirmed 133% increase in ammonia oxidisers in our Prosper trial. Drives the first stage of the nitrification cycle, converting ammonia into plant-available nitrite.
Nitrite oxidation: Confirmed 786% increase in nitrite oxidisers, including Nitrospira. Completes nitrification, locking nitrogen into the form plants can actually use.
Phosphate solubilisation: Bacillus megaterium unlocks phosphate bound in the soil matrix, making it available for plant uptake without additional applied phosphorus.
Trace mineral availability: Extracellular polysaccharide production by Bacillus megaterium mobilises trace minerals including zinc, iron, and manganese at the root surface.
Biological phosphorus removal: Glycogen accumulators including Defluviicoccus, confirmed present only in treated soil in the Prosper trial, support biological phosphorus cycling.
Soil aggregation: Filamentous bacteria increased 179% in treated soil. These organisms bind soil particles into stable aggregates, restoring the crumb structure that tillage and synthetic chemistry have systematically broken down. Stable aggregates are the architecture everything else depends on.
Pore space creation and maintenance: Healthy aggregation creates and preserves the macro and micro pores within the soil matrix. These spaces are not empty. They are the channels through which water moves, where air exchange occurs, and where roots extend. Compacted soil has lost them. The biology rebuilds them.
Water percolation: Bacillus subtilis biosurfactant activity and organic matter degradation restore the rate at which water moves through the soil profile. In degraded soil, rainfall sits on the surface and runs off. In biologically active soil with intact pore structure, it moves down into the profile where it can be held and used.
Aeration: Pore space is also the route through which oxygen reaches the root zone and CO₂ moves out. Compacted, biologically depleted soil suffocates roots and suppresses the aerobic microbial activity that drives nutrient cycling. Restored pore structure restores gas exchange, and with it the conditions that allow the rest of the biology to function.
Water infiltration: The combination of improved aggregation, restored pore channels, and biosurfactant activity dramatically increases the speed and volume of rainfall that enters the soil rather than leaving it as runoff. This is what Ray's jar test measures. It is the most visible indicator of whether a soil is alive or dead.
Water retention: Once water is in the profile, stable aggregates and increased organic matter hold it in the root zone for longer. The soil becomes a reservoir rather than a surface. Irrigation requirements fall. The crop has access to moisture through dry periods that would otherwise stress or kill it.
Heat tolerance: Bacillus-based formulations improve plant resilience to heat stress through enhanced root development and improved soil moisture availability around the root zone.
Drought tolerance: Deeper, more colonised root systems supported by the consortium access soil moisture further down the profile, giving crops better survival odds through dry spells.
Pathogen suppression: Bacillus subtilis produces subtilisin and biosurfactants that actively suppress soil-borne fungal and bacterial pathogens. Opportunistic pathogen populations were reduced by approximately 50% in treated soil in our Prosper trial.
Root colonisation and biomass: Enhanced root development through EPS production by Bacillus megaterium supports larger, more vigorous root systems and increased above-ground biomass.
Pesticide and herbicide degradation: Pseudomonas protegens, P. fluorescens, and P. chlororaphis degrade recalcitrant organics including legacy herbicide and pesticide residues that persist in conventionally farmed soils.
Persistent pollutant breakdown: Trametes sp. white-rot fungi deploy ligninolytic enzymes to break down persistent chemical compounds that bacterial species cannot reach, including certain industrial residues.
Heavy metal mobilisation: In combination with hemp phytoremediation, microbial activity mobilises heavy metals in the soil matrix, making them available for uptake and removal by the plant root system.

These functions do not operate in isolation. The value of a consortium over a single-strain inoculant is that the organisms work together. Nitrogen fixed by Microvirga becomes available through the nitrification pathway supported by Nitrospira, in soil whose structure is maintained by filamentous bacteria, in a root zone cleared of pathogens by Bacillus subtilis. The system restores itself. That is precisely what three decades of conventional management has prevented it from doing.

The root is where the
real data lives.

Conventional soil sampling tells you what is present in the bulk soil matrix. It is a useful baseline. But it is not where the biology that matters most is operating. The microbial community that directly governs plant health, nutrient uptake, and stress resilience lives in the rhizosphere, the narrow zone of soil immediately surrounding and attached to living plant roots. Bulk samples dilute that signal almost to nothing.

Within our field trials, we have the capability to extract root samples directly from the ground and run 16S rRNA sequencing on the rhizosphere community specifically. What comes back is a molecular picture of exactly which organisms are colonising the root system, what they are doing there, and how that community shifts in response to our inoculant. It is a level of diagnostic resolution that conventional agronomic soil testing does not approach.

Bulk soil tells you what is there
Standard soil sampling averages microbial populations across the entire soil volume. The organisms influencing the plant, living on and around the root surface, represent a fraction of that community and are functionally invisible in a bulk sample.
Root sampling tells you what is working
By sequencing the microbial community directly from root tissue and attached rhizosphere soil, we identify which species have successfully colonised the plant, at what abundance, and whether the functional traits we need, nitrogen cycling, phosphate solubilisation and pathogen suppression, are actually active at the root interface.
The difference in resolution
In our Prosper corn trial, 16S rRNA sequencing of treated soil confirmed Bacillus at 2.6% relative abundance and Nitrospira, a nitrite oxidiser, active in the community. Root-level sampling allows us to confirm whether those populations are colonising the plant itself, not just present in the surrounding soil.

What this means in practice

When we apply the consortium to a trial site, we are not guessing at whether the organisms established. We can pull roots, sequence them, and show exactly which species are present on the root surface and in what proportion, before and after treatment. That is the data layer that allows us to refine formulations with precision, publish peer-reviewed outcomes, and build the kind of scientific record that survives long after any single field season ends. It is also the data layer that most commercial inoculant programmes are not collecting.

A word on independence

The last company to develop a genuinely effective commercial microbial consortium was acquired by Bayer for $300M. Their formulations were quietly shelved. The product disappeared. The farmers who needed it never got it.

That is not what RAD Microbes is here to do. We are not building toward an exit. We are building toward the field, toward the farmer, toward the data, toward a published scientific record that cannot be bought and buried. The infrastructure around microbial agriculture is crowded with incumbents who benefit from the status quo. We intend to move through it regardless. The formulations work. The data will prove it. And the data will be public.

Two years in.
The data is building.

RAD Microbes is currently in its second year of active research and development. Our field work is grounded in real farm partnerships, genuine agronomic relationships, and measurable outcomes. This is not a laboratory proposition waiting for the field. We are already in the field.

Active: Year Two

University agronomic partnerships

Working relationships with agronomists from Texas A and M University and Louisiana State University, providing scientific rigour and institutional credibility to our field research programme across both states.

Active: This Season

Louisiana hemp farm trials

Multiple working farms in Louisiana are running our microbial formulations this season alongside university-coordinated test plots. These are live commercial operations on real ground, not controlled greenhouse studies.

Established

Dedicated microbial research farm: Stephen Thompson

Stephen Thompson has committed his farm as a dedicated microbial research site focused on industrial hemp cultivation alongside our formulation work. This gives us a controlled test plot environment that is not possible on commercial operations alone, with full data capture across the growing season.

Active partnership

Panda Biotech: hemp farming network

We are working with Panda Biotech, one of the world's largest industrial hemp extraction facilities, providing access to their farming network and seed supply as we scale our field programme across more operators and growing regions.

In discussion

Australian seed genomics collaboration

A developing relationship with Hemp Farms Australia is exploring hemp variety optimisation for Midwest growing conditions combined with our microbial formulation, identifying the most effective plant-biology pairings for large-scale soil remediation programmes.

Next phase

Pelletised delivery and biochar integration

Moving from liquid inoculant delivery toward a pelletised format incorporating biochar as a carrier medium, making application compatible with standard farm equipment and opening access to a wider range of conventional operators.

A note on the global picture. Soil degradation is not an American problem. The same conditions that are depleting the Midwest are at work across Central America, Sub-Saharan Africa, South Asia, and Australia. RAD Microbes is building a model that can be deployed internationally, making precision microbial tools available to farming economies where the soil health crisis is equally severe and the resources to address it are far fewer.

Ray, this is where
I need your help.

The work you have done through the Soil Health Academy and Understanding Ag has changed how farmers think about what is beneath their feet. You have taken something complex, the living economy of the soil, and made it plain enough that a farmer standing in his own field can see what is wrong and understand why it matters. That is a rare thing.

What you teach, we are building the tools to deliver. The science is there. The formulations are working. What we do not have, and what cannot be manufactured quickly, is the kind of long-standing trust with farming communities that makes a trial programme succeed. Getting farmers to stay the course across a full season, to resist the pull of conventional inputs when the biology is still establishing, to believe in something they cannot yet see in the yield data. That requires a relationship that was built over years, not months.

As you may know, I am English. I came here because I was called here. America is where this work can make the most significant difference, and I believe that with everything I have. What I do not yet have is the depth of relationship with farming communities across different states that this programme needs to grow. You do. And that is exactly what the next phase of this requires.

What I am asking is straightforward. We would supply our consortium to farmers within your existing network, at no cost to them, in exchange for their participation in structured trials. Your relationships carry the trust. We carry the science. The data we build together will be published, and what comes out of it belongs to the farming community. Not to a corporation, not to an acquirer, not to anyone who would bury it.

Access to your farming network
We are looking to run structured trials across multiple states and potentially internationally. Your relationships with farming communities, built across decades of work in the field, are the foundation we need. We supply the consortium at no cost. Your network provides the ground and the commitment to see the season through.
Equity in what we are building
This is not a consulting arrangement. We are asking you to be part of this from the ground up, and we want to reflect that properly. We are offering equity in Regenerative Agricultural Development LLC, a genuine stake in the outcome and not an honorarium. If this succeeds, it succeeds for both of us and for the farmers we are serving.
A shared conviction
We both believe the land was designed to heal itself if we stop working against it. That the biology was placed in the soil for a reason. That our job is not to engineer yield out of chemistry but to restore what was always meant to be there. The alignment between what you teach and what we are building is not coincidental. We think it is the point.

"The earth is the Lord's, and everything in it."

Psalm 24:1

We are stewards of it. Not owners. The work we are doing, returning living biology to degraded ground and giving farmers back something that was taken from them, feels, to both of us, like more than agronomy. We mention it not to preach, but because it is true, and because we think you will understand exactly what we mean.

radmicrobes.com
Confidential. This document has been prepared by Regenerative Agricultural Development LLC and is intended solely for Ray Archuleta. RAD Microbes™ is a trademark of Regenerative Agricultural Development LLC, Denton, Texas. Not for distribution without the written consent of RAD Microbes.